Standardized maximim D -optimal designs for enzyme kinetic inhibition models

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چکیده

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Potential application of D-optimal designs in the efficient investigation of cytochrome P450 inhibition kinetic models.

Correctly chosen d-optimal designs provide efficient experimental schemes when the aim of the investigation is to obtain precise estimates of parameters. In the current work, estimates of parameters refer to the enzyme kinetic parameters V(max) and K(m), but they also refer to the inhibition constant K(i). In general, this experimental approach is performed on a grid of values of the design var...

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A general model for enzyme kinetics with inhibition, the “mixed” inhibition model, simplifies to the non-competitive inhibition model when two of the parameters are equal. We reparamaterise the model and provide designs for investigating the equality of parameters, which corresponds to a scalar parameter δ being zero. For linear models T-optimum designs for discriminating between models in whic...

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Short Communication Potential Application of D-Optimal Designs in the Efficient Investigation of Cytochrome P450 Inhibition Kinetic Models

Correctly chosen D-optimal designs provide efficient experimental schemes when the aim of the investigation is to obtain precise estimates of parameters. In the current work, estimates of parameters refer to the enzyme kinetic parameters Vmax and Km, but they also refer to the inhibition constant Ki. In general, this experimental approach is performed on a grid of values of the design variables...

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Short Communication Potential Application of D-Optimal Designs in the Efficient Investigation of Cytochrome P450 Inhibition Kinetic Models

Correctly chosen D-optimal designs provide efficient experimental schemes when the aim of the investigation is to obtain precise estimates of parameters. In the current work, estimates of parameters refer to the enzyme kinetic parameters Vmax and Km, but they also refer to the inhibition constant Ki. In general, this experimental approach is performed on a grid of values of the design variables...

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Short Communication Potential Application of D-Optimal Designs in the Efficient Investigation of Cytochrome P450 Inhibition Kinetic Models

Correctly chosen D-optimal designs provide efficient experimental schemes when the aim of the investigation is to obtain precise estimates of parameters. In the current work, estimates of parameters refer to the enzyme kinetic parameters Vmax and Km, but they also refer to the inhibition constant Ki. In general, this experimental approach is performed on a grid of values of the design variables...

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ژورنال

عنوان ژورنال: Chemometrics and Intelligent Laboratory Systems

سال: 2017

ISSN: 0169-7439

DOI: 10.1016/j.chemolab.2017.08.009